NEW RI PLASMID T-DNA MOLECULE AND VECTOR CONTAINING AT LEAST PART OF NEW RI PLASMID T-DNA

摘要

NEW MATERIAL:Ri plasmid T-DNA molecule containing at least part of fragment of T-DNA region of Ri plasmid, i.e., plasmid pRi 1724 which Agrobacterium rhizogenes MAF 03-1724 strain has. USE:Prove for proving plant transformation and plant transformation vector. PREPARATION:For example, Ri plasmid which Agrobacterium rhizogenes MAF 03-1724 strain has is cut into various DNA fragments with restriction enzymes to bind the DNA fragment to a vector and then the vector is introduced into Escherichia coli to prepare DNA library and clones containing a desired T-DNA region is selected by hybridization from among these clones and cultured to provide the Ri plasmid T-DNA molecule from among recombinant vector.