POTATO HAIRY ROOT CAPABLE OF PRODUCING 5-LIPOXYGENASE, ITS PRODUCTION AND 5-LIPOXYGENASE

摘要

PURPOSE:To enable the stable production of lipoxygenase in a large amount without receiving fluctuation of activity due to a difference in production areas of potato tubers by culturing hairy roots developed by transforming tissues of potato with a specific microorganism. CONSTITUTION:Agrobacterium rhizogenes is inoculated into tissues from a tuber, stem, leaf part, etc., of a plant potato capable of producing 5-lipoxygenase and transformation is carried out to induce hairy roots. The resultant hairy roots are then cultured in an inorganic culture medium containing a saccharide, amino acid, vitamin, etc., without containing phytohormone added thereto to afford hairy roots having 5-lipoxygenase activity. The hairy roots are subsequently pulverized in an acetic acid buffer solution and filtered. Ammonium sulfate is then added to the resultant supernatant and 5-lipoxygenase is precipitated and obtained.