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method for introduction of foreign dna into the genome of dicotylen plants; a process for the manufacture of agrobacterium tumefaciens transformation of bacteria.
method for introduction of foreign dna into the genome of dicotylen plants; a process for the manufacture of agrobacterium tumefaciens transformation of bacteria.
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机译:将外源DNA导入双子叶植物基因组的方法;用于生产根癌农杆菌转化细菌的方法。
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摘要
The invention relates to a process for the incorporation of foreign DNA into the genome of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium tumefaciens bacteria, which contain one or more Ti (tumour inducing) plasmids, wherein as Ti plasmid a stable cointegrate plasmid composed of the plasmid R772 and the plasmid pTiB6 with foreign DNA incorporated in the T-region of the Ti component of the cointegrate plasmid is applied as well as to a cointegrate plasmid pAL969 and cointegrate plasmids derived from the cointegrate plasmid by the incorporation of foreign DNA into the T-region of the Ti component.
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