DERIVATISATION REAGENTS FOR USE IN THE HPLC-ECD ANALYSIS OF PEPTIDES AND PROTEINS

摘要

The invention concerns the use of hydrolysis- and air-stable metal-aromatic complexes, metal-carbonyl-aromatic complexes or metalcarbonyl-carbene complexes with substituents which are reactive towards a terminal or side-chain amino group of a protein. By reacting peptides or proteins with these reagents, these classes of compound can be made suitable for analysis by high-pressure liquid chromatography using an electro-chemical detector. Preferred reagents are ferrocene complexes such as 3-ferrocenylsuccinimidyl propionate, 3-ferrocenylpropionic acid anhydride, ferrocenylmethyl isocyanate and N-ferrocenylmethylsuccinimidyl glycine hydrochloride.