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Optimized fermentation processes for the production of foreign proteins en E-coli
Optimized fermentation processes for the production of foreign proteins en E-coli
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机译:优化发酵工艺以生产大肠杆菌中的外源蛋白
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摘要
The invention describes optimised fermentation processes for the production of foreign proteins in E.coli using the lac promoter or improved lac promoter (e.g. tac, trc). After the initial growth phase with glucose as carbon source, product formation is induced (1) by IPTG with glucose limitation or (2) by lactose with lactose limitation or (3) by IPTG and lactose with lactose limitation. The limitation of glucose or lactose is such that the partial pressure of oxygen remains above 10%.
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