Enhanced expression of heterologous proteins in recombinant hosts in a non-growth media

摘要

The present invention relates to a fermentation process for producing recombinant proteins in a recombinant host strain. The process can be adapted for organisms containing a mutation in cell wall and/or plasma membrane biosynthesis (e.g. mnn9) in a non-growth media. An example of such a strain is described in Merck's Application serial number 202,764, filed June 3, 1988, corresponding to EP-­A-0344864. Additionally, the process can be adapted for organisms containing "wild-type" cell walls and plasma membrane.