STRAIN OF CULTURED ANIMAL CELLS OF MUS MUSCULUS L EMPLOYED FOR THE PRODUCTION OF A HYBRIDOME

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to u0433u0438u0431u0440u0438 - u0434u043eu043cu043du043eu0439 technology and can be used to obtain a hybrid. the purpose of u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 is a strain of tumor cells - partners with a reduced dependence on u043cu0430u043au0440u043eu0444u0430u0433u0430u043bu044c for u0444u0438u0434u0435u0440u0430, high speed proliferation and u0432u044bu0445u043e house is a hybrid. the strain obtained from the cells of sp 2 / 0 by selection for resistance to methyl u044du0442u0438u0446u0438u044e (ev) in a concentration of 5 mg / ml).the fish in the environment u0434u043cu0435u043c with 10% serum, fetal cows, 1 mm of amino acids, u043fu0438u0440u0443u0432u0430u0442u0430 u043du0430u0442u0440u0438u00a0 1 mm, 2 mm u0433u043bu044eu0442u0430u043cu0438 -, u043cu0435u0440u043au0430u043fu0442u043eu044du0442u0430u043du043eu043bu0430 and 40 iu / ml u0433u0435u043du0442u0430u043c u0438u0446u0438u043du0430. u043fu0442u0430u043cu043c u043eu0442u043bu0438u0447u0430u0435u0442u0441u00a0 source u043fu043eu00a0u0432u043bu0435u043du0438u0435u043c from at least two chromosomal markers, resistance to ev, as well as to 0.5 mg / ml of colchicine and 0074 micrograms / ml u0430u043au0442u0438u043du043eu043cu0438u0446u0438u043du0430 d.u0433u0438u0431u0440u0438u0434u043eu043cu044b obtained during hybridization lymphocytes mice with cells u0437u0430u00a0u0432u043bu00a0u0435u043cu043eu0433u043e strain grown without u0434u043eu0431u0430u0432u043bu0435u043du0438u00a0 monocyte derived macrophages and exit u043fu0440u043eu0434u0443u0446u0435u043du0442u043eu0432 antibodies, double than with the use of the prototype sp 2 / 0 ag 14, the strain is deposited with the no. u0432u0441u043au043a (p) 309u0434. about $