STRAIN OF HYBRID CULTURED ANIMAL CELLS OF MUS MUSCULUS L USED FOR PRODUCTION OF MONOCLONAL ANTIBODIES TO CHLAMYDIA

摘要

FIELD: veterinary biotechnology. SUBSTANCE: strain Chla-K-1 is obtained by fusing mouse myeloma cells NSO with splenocyte cells of BALB/c mouse line immunized by elementary corpuscles Chlamydia psittaci prepared from strain "K-1" feline chlamydiosis conjunctivitis exciter. Hybridoma Chla-K-1 produces genus-specific Gl isotype monoclonal antibodies reacting with different agents exciting feline, cheep, fur-bearing animal, and human chlamydiosis. Activity of antibodies in cultural fluid ranges from 1:2000 to 1:6000, in ascitic fluid from 1:200000 to 1:1000000. Antibodies are revealed in immunoenzyme test involving immuno-fluorescence. EFFECT: enabled preparation of express diagnosticum for detection of Chlamydia genus detection. 1 tbl, 4 ex