METHOD immunosorbent assay for determining von Willebrand factor, a monoclonal antibody to von Willebrand factor (Options) and HYBRID STRAIN cultured animal cells MUS. MUSCULUS L.- PRODUCER monoclonal antibodies to von Willebrand factor (optional)

摘要

1.lateral flow screening method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 willebrand factor, including u0441u0435u0431u00a0 u0441u043eu0440u0431u0446u0438u044e willebrand antibodies to factor in the hole board, u043eu0442u043cu044bu0432u043au0443 u043du0435u0441u043eu0440u0431u0438u0440u043eu0432u0430u0432u0448u0438u0445u0441u00a0 ant u0438u0442u0435u043b buffering solution, the hole u0430u043du0430u043bu0438u0437u0438u0440u0443u0435u043cu0443u044e sample containing willebrand factor, u0438u043cu043cu0443u0431u0438u043bu0438u0437u0430u0446u0438u044e willebrand factor, the samples u0434u0435u0442u0435u043au0442u0438u0440u0443u044eu0449u0435u0433u043e reagent based on antibodies.processing of samples u0445u0440u043eu043cu043eu0433u0435u043du043du044bu043c substrate and measurement of outcomes using spectroscopy, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0, as u0438u043cu043cu043eu0431u0438u043bu0438u0437u0443u044eu0449u0435u0433u043e antibodies using mon u043eu043au043bu043eu043du0430u043bu044cu043du043eu0435 5u04413 antibody, as well as u0434u0435u0442u0435u043au0442u0438u0440u0443u044eu0449u0435u0433u043e reagent mixture u0431u0438u043eu0442u0438u043du043eu043c marked monoclonal antibodies 2H2 and 7D12.;2. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that a u0434u0435u0442u0435u043au0442u0438u0440u0443u044eu0449u0435u0433u043e reagent used u043cu0435u0447u0435u043du043du043eu0435 u0431u0438u043eu0442u0438u043du043eu043c 2u043d2 antibody.;3. method for u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 1, so that a u0434u0435u0442u0435u043au0442u0438u0440u0443u044eu0449u0435u0433u043e reagent used u043cu0435u0447u0435u043du043du043eu0435 u0431u0438u043eu0442u0438u043du043eu043c 7D12 antibody.;4. u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0435 antibody 5u04413, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0435u0435u0441u00a0, u043fu0440u043eu0434u0443u0446u0438u0440u0443u0435u0442u0441u00a0 strain u0433u0438u0431u0440u0438u0434u043eu043cu043du044bu0445 cultured cells Mus musculus l. 5C3, u043eu0442u043du043eu0441u0438u0442u0441u00a0 class IgG1, sent about rets willebrand factor and competes for the u0441u0432u00a0u0437u044bu0432u0430u043du0438u0435 with willebrand factor with antibodies 2u043d2 and 7D12.;5. u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0435 antibody 2u043d2, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0435u0435u0441u00a0, u043fu0440u043eu0434u0443u0446u0438u0440u0443u0435u0442u0441u00a0 strain u0433u0438u0431u0440u0438u0434u043eu043cu043du044bu0445 cultured cells Mus musculus l. 2H2, u043eu0442u043du043eu0441u0438u0442u0441u00a0 class lgG2b, sent about rets willebrand factor and competes for the u0441u0432u00a0u0437u044bu0432u0430u043du0438u0435 with willebrand factor with antibodies 5C3 and 7D12.;6. u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0435 antibody 7D12, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0435u0435u0441u00a0, u043fu0440u043eu0434u0443u0446u0438u0440u0443u0435u0442u0441u00a0 strain u0433u0438u0431u0440u0438u0434u043eu043cu043du044bu0445 cultured cells Mus musculus l. 7D12 u043eu0442u043du043eu0441u0438u0442u0441u00a0 class IgGI, sent about rets willebrand factor and competes for the u0441u0432u00a0u0437u044bu0432u0430u043du0438u0435 with willebrand factor with antibodies 5C3 and 2H2.;7. hybrid strain to cultured cells Mus musculus l. 5C3, u0434u0435u043fu043eu043du0438u0440u043eu0432u0430u043du043du044bu0439 in u0440u043au043au043a (u2116u0440u043au043au043a (p) 684u0434) used as a producer u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0433u043e antibodies against factor 5C3 villas u0435u0431u0440u0430u043du0434u0430.;8. hybrid strain to cultured cells Mus musculus l. 2H2, u0434u0435u043fu043eu043du0438u0440u043eu0432u0430u043du043du044bu0439 in u0440u043au043au043a (u2116u0440u043au043au043a (p) 683u0434) used as a producer u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0433u043e antibodies against factor 2H2 villas u0435u0431u0440u0430u043du0434u0430.;9. hybrid strain to cultured cells Mus musculus l. 7D12, u0434u0435u043fu043eu043du0438u0440u043eu0432u0430u043du043du044bu0439 in u0440u043au043au043a (u2116u0440u043au043au043a (p) 685u0434) used as a producer u043cu043eu043du043eu043au043bu043eu043du0430u043bu044cu043du043eu0433u043e antibodies against factor 7D12 wheel u043bu0435u0431u0440u0430u043du0434u0430.