STRAIN OF HYBRID CULTURED ANIMAL CELLS MUS MUSCULUS L EMPLOYED FOR PREPARING MONOCLONAL ANTIBODIES TO NUCLEOPROTEID STRUCTURAL PROTEIN OF RABIES VIRUS

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to u0433u043du0431u0440u0438 - u0434u043eu043cu043du043eu0438 technology and can be used u0434u043bu00a0 diagnosis of rabies virus (wb). purpose to obtain a hybrid u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 cultured cells of animals Mus musculus l., producing monoclonal antibodies to u043du0443u043au043bu0435u043eu043fu0440u043eu0442u0435u0438u0434u043du043eu043cu0443 structurally mu squirrel wb.u0433u0438u0431u0440u0438u0434u043eu043cu0443 receive u0441u043bu0438u00a0u043du0438u0435u043c messenger substances, with which Sp2 / 0 cells of murine spleen cells u043cu044bu0448u0435u0438 Balb / c immunized wb figure 12. u043cu0442u0430u043cu043c hybrid cells was named crooked - u043b7 and deposited with the number u0432u0441u043au043a (ii) no. 325u0408. standard u0443u0441u043bu043eu0432u0438u00a0 u043au0443u043bu044cu0442u0438u0432u0438u0440u043eu0432u0430u043du0438u00a0 strain environment u0434u0443u043bu044cu0431u0435u043au043au043e with 20 percent of embryonic u0442u0435u043bu00a0u0447u044cu0435u0439 serum, 2u043cu043c u0433u043bu044eu0442u0430u043cu043du043du0430 and 50 micrograms / ml u0433u0435u043du0442u0430u043cu0438 - u0446u0438u043du0430.the frequency of u043fu0430u0441u0441u0438u0440u043eu0432u0430u043du0438u00a0 through 23u0441u0443u0442. u043fu043eu0441u0435u0432u043du0430u00a0 dose of 150000 cells in 1 ml. shot 1: adding 2 1: 3. the strain produced u043cu043eu043du0430u0442 class igg 1, titer u043cu043eu043du0430u0442 u0434u043bu00a0 u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 fluid u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 1, 270 and 1: 7290 u0434u043bu00a0 u0430u0441u0446u0438u0442u043du043eu0439 fluid. u043cu043eu043du0430u0442 specifically react with protein u043du0443u043au043bu0435u043eu043fu0440u043eu0442u0435u043du0434u043du044bu043c wb in the reaction u043du0435u043fu0440u00a0u043cu043eu0439 immune boosting u0444u043bu044eu043eu0440u0435u0441u0446u0435u043du0446u0438u0438 (u21161u0444u0430) and u043du0435u043fu0440u00a0u043cu043eu0439 reaction immune enzyme analysis (u043du043du0444u0430). 1, table 2).5u044cu0437u0445u043au044d of medi.