METHOD FOR PREPARATION OF CDNA-CLONES, INCODING HUMAN ERYTHROPOIETIN

摘要

FIELD: genetic engineering. SUBSTANCE: invention relates to human erythropoietin gene cloning. Mammalian cells transformed by the recombinant vector are cultured in suitable medium. Vector has DNA sequences given in tables 3 and 4. DNA sequence can be introduced into vector containing DNA of bovine papilloma virus. Mammalian cells are lines COS, CHO, C127 or 3T3. Cultural acid can contain fetal serum. Obtained erythropoietin is separated from cells and medium. Method ensures to obtain the cloned human erythropoietin genes exhibiting highly levels of expression. EFFECT: high levels of expression of the cloned erythropoietin genes. 6 cl, 11 tbl, 12 dwg, 15 ex