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Study of Immunoassay Methods for Recombinant Human Erythropoietin(rhEPO)Using Competitive ELISA

机译:竞争性ELISA技术用于重组人促红细胞生成素(rhEPO)免疫测定方法的研究

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摘要

Two different immunoassay methods, competitive indirect enzyme-linked immuno- sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO.The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively.The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.
机译:研究了两种不同的免疫测定方法,竞争间接酶联免疫吸附测定(CI-ELISA)和放大竞争间接间接ELISA(ACI-ELISA),使用生物素-亲和素复合物系统检测rhEPO。线性范围为50-20000 ng / CI-ELISA和ACI-ELISA分别为10 mL和10-50000 ng / mL.CI-ELISA和ACI-ELISA的低检测限分别为62.8 ng / mL和8.5 ng / mL。

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