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DNA coding for antigen protein of rinderpest virus

机译:牛瘟病毒抗原蛋白的DNA编码

摘要

A DNA coding for an entire or a part of an antigen protein of the rinderpest virus; a protein for the production of the DNA comprising the steps of preparing an mRNA from the rinderpest virus, preparing a cDNA library from the mRNA, selecting a cDNA coding for the target protein from the cDNA library, and cloning the selected cDNA in a cloning vector; and a protein for the production of the antigen protein of the rinderpest virus comprising the steps of transfecting a vector containing the DNA coding for the target protein into animal cells, culturing the animal cells to produce the antigen protein, and recovering the target protein from the cell culture.
机译:编码牛瘟病毒全部或部分抗原蛋白的DNA;用于产生DNA的蛋白质,其包括以下步骤:从牛瘟病毒制备mRNA,从该mRNA制备cDNA文库,从cDNA文库中选择编码目标蛋白的cDNA,并将所选择的cDNA克隆在克隆载体中;用于产生牛瘟病毒抗原蛋白的蛋白质,其包括以下步骤:将含有编码靶蛋白的DNA的载体转染到动物细胞中,培养动物细胞以产生抗原蛋白,并从中回收靶蛋白。细胞培养。

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