ANALYZING METHOD FOR ASCORBIC ACID AND DEHYDROASCORBIC ACID

摘要

PURPOSE:To perform analysis with excellent selectivity in high sensitivity by separating ascorbic acid and dehydroascorbic acid by high-speed liquid chromatography, making the eluate from a column basic, and detecting the reaction product by a absorbance method. CONSTITUTION:The liquid in a moving phase 9 is sent with a liquid sending pump 1. A sample containing ascorbic acid and dehydroascorbic acid is introduced from a sample introducing device 3 with a micro-syringe and the like. Both components are separated with a column 8. Alkali solution 10 containing a reducing agent is sent and mixed into the moving-phase liquid which is continuously eluted from the column with a liquid sending pump 2. The mixture is sent into a reactor 7. Here, both components are guided into a compound having the maximum absorption at 300 nm and detected with an absorbance detector 5. The result is processed in a data processor 6.