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METHOD AND REAGENT FOR MEASURING QUANTITY OF ASCORBIC ACID AND DEHYDROASCORBIC ACID
METHOD AND REAGENT FOR MEASURING QUANTITY OF ASCORBIC ACID AND DEHYDROASCORBIC ACID
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机译:测定抗坏血酸和脱氢抗坏血酸数量的方法和试剂
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摘要
Ascorbic acid or dehydroascorbic acid is determined by reaction with a tetrazolium salt selected from 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide, nitrotetrazolium blue, tetrazolium blue chloride and distyryl-nitro blue tetrazolium chloride, in the presence of phenazine methosulphate and a detergent, at a weakly acidic pH value, and evaluating the coloration obtained as a measure of the initial acid content. The method is particularly efficacious in substantially eliminating disturbance of the reaction by foreign substances.
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