TISSUE PLASMINOGEN ACTIVATOR ACTIVITY MEASUREMENT USING ANTIBODY WITH AFFINITY TO SITE OTHER THAN ACTIVE SITE OF TPA

摘要

True activity of tissue plasminogen activator is determined by reacting a solid phase having bound thereto anti tissue plasminogen activator antibody IgG fragment having affinity to a site other than the active site of tissue plasminogen activator with tissue plasminogen activator in a sample and then measuring activity of the reaction product, preferably in terms of amount of substance released from a chromogenic peptide substrate. This measurement enables use of whole blood or diluted blood as the sample and achieves high sensitivity and high accuracy in a short period of time.