This invention relates to the manufacture of ethanol and the recovery of such by-products therefrom as glycerol, betaine, succinic and lactic acid, potassium sulfate and a free flowing distiller's dry grain and solubles useful as an animal feed or a component of food for humans or as a premix for agricultural fertilizer. The process disclosed is an improvement over that of U.S. application Ser. No. 136,415 in the area of enhancement of glycerol recovery from stillage resulting from the fermentation of a biomass mash, and involves production of glycerol in amount in excess of 10 grams per 100 grams of reducing sugar in the starting biomass mash.
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