PURPOSE: To provide an ultra-thin antibody immobilized film enhanced in antibody density and having high reactivity and high sensitivity, that is, rapid response and to obtain a biosensor, a bioreactor, a bioelectronics device and an immunoassay substrate from said film. ;CONSTITUTION: An aqueous solution of protein A is dropped and developed on the surface of the water to be transferred to a glass substrate and antibody protein (anti-human serum albumin antibody) is allowed to act on the formed built-up film of protein A to immobilize antibody protein and a biosensor is formed using the obtained ultrathin antibody immobilized film. This antibody immoblized film is easily obtained by simple operation and, since the antibody imobilized film bonds the FC region of an antibody to a protein A.LB film, the antigen confirming region of the antibody is turned to the outside and, by changing an antibody, antibody films to various antigens can be formed and a sensor obtained using the antibody immobilized film can be easily reacted with an antigen to be measured.;COPYRIGHT: (C)1993,JPO&Japio
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