IMMOBILIZATION OF ANTIBODY PROTEIN WITH LIPID MONOMOLECULAR FILM

摘要

PURPOSE:To densely immobilize the titled substance useful as a diagnostic material or the like, by bringing the antibody protein dissolved in an aq. phase into contact with a long chain fatty acid monomolecular film developed on the surface of the aq. phase to form an antibody protein-monomolecular film complex at the interface and then transferring the formed complex to a solid substrate. CONSTITUTION:A 24-32C long chain fatty acid (e.g., lignoceric acid), a polyvalent metal salt thereof and/or an ester thereof are dissolved in a solvent such as chloroform and then gradually dripped on a water bath filled with distilled water to form a film developed on the surface of the water. A dividing plate placed on the water surface of the water bath is moved until the surface pressure becomes 20mN/m. Subsequently, an aq. soln. of a water-soluble antibody protein is poured into the water bath to bring the antibody protein into contact with the monomolecular film formed on the surface of the aq. phase to form an antibody protein-monomolecular film complex at the interface of the aq. phase. The formed complex is transferred to a solid substrate to immobilize the antibody protein with the lipid monomecular film.