Derivatization of Polystyrene and Nylon Beads. A Controlled Introduction of Functional Groups for Immobilization of Antibody Protein

摘要

An attempt was made to modify the surface properties of nylon balls and polystyrene tubes so that protein could be covalently attached. Using 25I labeled antibody as our probe to quantitate the amount of protein bound to the solid support, the modified solid supports immobilized from 2-5 times more antibody than the non-modified supports. Also, our data show that glutaraldehyde was 2-4 times more effective than carbodiimide as a coupling reagent for antibody immobilization. It is apparent that receptors for antibody protein on either the modified or unmodified supports remain free or unbound at every level of antibody protein concentration used to sensitize the supports, which in practice, permits nonspecific uptake of other protein molecules subsequently used in reaction identification. This causes difficulty in test interpretation. Work is in progress to determine if the unbound receptor sites can be effectively blocked without reducing the immunoreactivity of the immobilized antibody.