IMMOBILIZED GLUCOSE OXIDASE ENZYME MEMBRANE AND PRODUCTION THEREOF

摘要

PURPOSE:To obtain an immobilized glucose oxidase enzyme membrane necessary for continuous use of glucose oxidase and applicable to a biosensor, by using polyvinyl pyrrolidone and bovine serum albumin, etc., as raw materials. CONSTITUTION:An immobilized glucose oxidase enzyme membrane is formed by coating a substrate with an aqueous solution containing 2-20wt% polyvinyl pyrrolidone having a molecular weight of several tens thousand - several hundred thousand, 0.5-1.5wt% 2,5-bis(4'-azido-2'-sulfobenzal)cyclopentanone sodium salt, 5.0-7.5wt% glucose oxidase and 5-10wt% bovine serum albumin and drying the coating layer. The immobilizing enzyme membrane is crosslinked by ultraviolet irradiation and then chemically crosslinked by treating with a chemical crosslinking agent such as an aqueous solution of glutaraldehyde.