A membrane and the method of making the same is disclosed for use with glucose analyzers to detect the level of glucose in liquid mediums such as whole blood. The membrane comprises a thin base wafer of a hydrophobic fluorocarbon material such as polytetrafluoroethylene (TEFLON). The wafer is initially etched by removing surface fluorides to produce a hydrophilic surface and is then contacted with a mixed reagent formed by combining in a volume ratio of about 1:1:1 about 4% paraformaldehyde in water, about 5% bovine serum albumin in water and about 10% glucose oxidase in water. The resultant membrane contains glucose oxidase covalently bonded to the surface of the base wafer.
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