PRODUCTION OF ALGINIC ACID OLIGOSACCHARIDE

摘要

PURPOSE:To efficiently obtain an alginic acid oligosaccharide useful for increasing yield of agricultural crops, by reacting a substance containing alginic acid (salt) with alginate lyase and terminating the reaction when the reaction solution attains a given absorbance. CONSTITUTION:Alteromonas sp. LB 102 strain (FERM P-9218) which is a microorganism of the genus Alteromonas is cultivated and the resultant culture is extracted to provide alginate lyase (A) having about 7 optimum pH and about 40 deg.C optimum temperature. The resultant enzyme (A) in an amount of 100-4,000 units based on 1g sodium alginate is then added to a solution (B) prepared by adding 100pts. water to 1-5pts. sodium alginate and adjusting the pH to about 7 and reacted at about 40 deg.C for 24-48hr. When the absorbance at 230nm (E1cm1) attains 30-60, the reaction is terminated to adjust the pH of the reaction solution to 1-4. Heat treatment is carried out at 100-120 deg.C for 15-180min to produce the aimed alginic acid oligosaccharide.