PURIFICATION OF ALGINIC ACID OLIGOSACCHARIDE AND SULFURIC ACID GROUP-CONTAINING OLIGOSACCHARIDE

摘要

PURPOSE:To extremely readily fractionate an oligosaccharide by the once employment of a chromatography comprising a HPLC using an anion exchange resin column. CONSTITUTION:A method for purifying an oligosaccharide containing an alginic acid oligosaccharide or a sulfuric acid group-containing polysaccharide is characterized by making the alginic acid oligosaccharide prepared from alginic acid or a polysaccharide containing sulfuric acid groups in the structure of the alginic acid oligosaccharide adsorbed onto an anion exchange resin column and then applying to a high performance liquid chormatography to fractionate the oligosaccharide by a concentration gradient elution method using water and sodium chloride.