TYROSINE-O-SULFATE BINDING PROTEIN AND METHOD FOR ENHANCING ACTIVITY OF SULFATE TRANSFERASE

摘要

PURPOSE:To obtain the title protein useful as a medicine for promoting metabolism, etc., derived from serum, bonded to carbonyl group-free tyrosine sulfate Affitystar, being eluted with 1M aqueous solution of salt and linked to tyrosine- O-sulfate. CONSTITUTION:After a carboxyl group-free tyrosine sulfate Affitystar column is equilibrated with physiological sodium chloride solution buffered with phosphoric acid, bovine serum, etc., is introduced through the column and subjected to affinity chromatography, then, the column is cleaned, 1 M aqueous solution of salt is dropped through the column and an adsorbent is eluted to give the objective protein which has 170 kDa molecular weight by SDS-PAGE method under a reducing condition and 160 kDa under a nonreducing condition, and the protein bonds to tyrosine-O-sulfate and tyrosine-O-phosphate but not to glycine, tyrosine, gelatin and protein A and does not immunologically cross with tyrosine sulfated protein receptor antibody, anti-human alpha-2 macroglobulin antibodym anti-human complex C3 antibody and anticomplex 4C antibody.