Induction of Calcium Flux and Enhancement of Cytolytic Activity in Natural Killer Cells by Cross-Linking of the Sheep Erythrocyte Binding Protein (CD2) and the Fc-Receptor (CD16)

摘要

Binding of the anti-cluster of differentiation (CD)2 monoclonal antibody 9-1 causes an increase in the concentration of cytoplasmic-free calcium ((Ca(2+)i) in cultured CD3(-)/CD16(+) natural killer (NK) cells. This response did not occur in cultured CD3(+)/CD16- cytotoxic T lymphocytes (CTL). Anti-CD16 antibodies could partially block the calcium response when NK cells were stimulated with intact antibody 9-1, and antigen-binding fragment F(ab')2 of antibody 9-1 did not produce a calcium response. Thus an interaction of the 9-1 antibody with CD16 Fc receptors was required for the functional effect. The cytolytic activity of cultured NK cells was increased by antibody 9-1 but not by F(ab')2 fragments of antibody 9-1. The enhanced lytic activity was blocked by anti-CD16 antibody, anti-CD18 antibody, and anti-CD2 antibodies that do not block the binding of antibody 9-1. This pattern was distinct from antibody-dependent cell-mediated cytotoxicity which was blocked only by the anti-CD16 antibody. Thus antibody 9-1 enhanced cytotoxicity by activating effector cells. There was no enhancement of lytic activity when F(ab')2 of antibody 9-1 were cross-linked with a polyclonal antiglobulin, even though (Ca(2+))i was increased. These results show that induction of a (Ca(2+))i response is not sufficient to enhance lytic activity in NK cells, and suggest that signals delivered through CD16 are necessary. Keywords: Immunology; Reprints. (aw)