IMMUNOLOGICAL METHOD FOR DETERMINATION OF HUMAN 92KDA GELATINASE

摘要

PURPOSE: To determine human 92kDa gelatinase (human MMP-9) in a sample to be inspected, precisely, simply and quickly, by using a monoclonal antibody which is combined specifically with the human MMP-9. ;CONSTITUTION: Two kinds of monoclonal antibodies which are combined specifically with human MMP-9 are used as antibodies to be combined with a solid- phase carrier or antibodies giving tags thereto, and measurement is executed immunologically by a sandwich method. On the occasion when an antigen- antibody reaction is executed, polyoxyethylene lauryl alcohol ether is added to a reaction liquid preferably in a concentration range of 0.02 to 0.1% (W/V) generally. Thereby nonspecific combination and interference in the antigen- antibody reaction by serum protein can be lessened and, therefore, the human MMP-9 in a specimen can be measured with increased accuracy.;COPYRIGHT: (C)1993,JPO&Japio