首页> 外国专利> AVIAN-DERIVED ANTIBODY CAPABLE OF BINDING SPECIFICALLY TO HUMAN HMGB1, IMMUNOLOGICAL DETERMINATION METHOD FOR HUMAN HMGB1, AND IMMUNOLOGICAL DETERMINATION REAGENT FOR HUMAN HMGB1

AVIAN-DERIVED ANTIBODY CAPABLE OF BINDING SPECIFICALLY TO HUMAN HMGB1, IMMUNOLOGICAL DETERMINATION METHOD FOR HUMAN HMGB1, AND IMMUNOLOGICAL DETERMINATION REAGENT FOR HUMAN HMGB1

机译：能够与人HMGB1特异性结合的禽类抗体，人HMGB1的免疫学测定方法以及人HMGB1的免疫学测定试剂

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摘要

An object of the present invention is to provide an anti-human HMGB1 antibody having high capability of binding to human high mobility group box protein-1 (HMGB1) with a high probability. According to the present invention, a highly sensitive immunoassay method and immunoassay reagent for human HMGB1 in a sample using the anti-human HMGB1 antibody are provided. Specifically, provided herein are: an avian-derived anti-human HMGB1 antibody specifically binding to the amino acid sequence shown by the following formula (I): Lys Pro Asp Ala Ala Lys Lys Gly Val Val Lys Ala Glu Lys Ser (I) of human HMGB1; and an immunoassay method and an immunoassay reagent for human HMGB1 in a sample, which are characterized by using the antibody.

机译：本发明的目的是提供具有高可能性与人高迁移率族盒蛋白-1（HMGB1）结合的能力高的抗人HMGB1抗体。根据本发明，提供了使用抗人HMGB1抗体的样品中的人HMGB1的高度灵敏的免疫测定方法和免疫测定试剂。具体地，本文提供：与下式（I）所示的氨基酸序列特异性结合的禽源抗人HMGB1抗体：Lys Pro Asp Ala Ala Ala Lys Lys Gly Val Val Lys Ala Glu Lys Ser（I）人HMGB1; 使用该抗体表征的样品中人HMGB1的免疫测定方法和免疫测定试剂。 展开▼

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公开/公告号EP2105447A1

专利类型

公开/公告日2009-09-30

原文格式PDF

申请/专利权人 SHINO-TEST CORPORATION;
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申请/专利号EP20070860273

发明设计人 YAMADA SHINGO;YAKABE KEIKO;
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申请日2007-12-19

分类号C07K16/18;G01N33/53;

国家 EP

入库时间 2022-08-21 19:14:50

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1. 用于神经病症的预后的HMGB1和抗HMGB1抗体 [P] . 中国专利： CN102884432B . 2016.01.13

2. 用于神经病症的预后的HMGB1和抗HMGB1抗体 [P] . 中国专利： CN102884432A . 2013-01-16

3. AVIAN-DERIVED ANTIBODY CAPABLE OF BINDING SPECIFICALLY TO HUMAN HMGB1, IMMUNOLOGICAL DETERMINATION METHOD FOR HUMAN HMGB1, AND IMMUNOLOGICAL DETERMINATION REAGENT FOR HUMAN HMGB1 [P] . 欧洲知识产权局专利： EP2105447A4 . 2010-05-05

机译：能够与人HMGB1特异性结合的禽类抗体，人HMGB1的免疫学测定方法以及人HMGB1的免疫学测定试剂

4. AVIAN-DERIVED ANTIBODY CAPABLE OF BINDING SPECIFICALLY TO HUMAN HMGB1, IMMUNOLOGICAL DETERMINATION METHOD FOR HUMAN HMGB1, AND IMMUNOLOGICAL DETERMINATION REAGENT FOR HUMAN HMGB1 [P] . 美国专利： US2009297546A1 . 2009-12-03

机译：能够与人HMGB1特异性结合的禽类抗体，人HMGB1的免疫学测定方法以及人HMGB1的免疫学测定试剂

5. AVIAN-DERIVED ANTIBODY CAPABLE OF BINDING SPECIFICALLY TO HUMAN HMGB1, IMMUNOLOGICAL DETERMINATION METHOD FOR HUMAN HMGB1, AND IMMUNOLOGICAL DETERMINATION REAGENT FOR HUMAN HMGB1 [P] . 世界知识产权组织专利： WO2008075788A1 . 2008-06-26

机译：能够与人HMGB1特异性结合的禽类抗体，人HMGB1的免疫学测定方法以及人HMGB1的免疫学测定试剂

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1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004）（P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期

2. HMGB1 and HMGB2 Expression Patterns in Human Papillary Renal Cancers [J] . Takumi Takeuchi ,Koichi Sakazume ,Akiko Tonooka . 泌尿学期刊（英文） . 2013,第1期

3. Effects of HMGB1 Expression Suppressed by siRNA on Cell Cycle and Proliferation of Human Cervical Cancer Cell Line HeLa [J] . Yuan-yuan QIU ,Hui-yu WANG ,Quan HAO . 临床肿瘤和癌症研究 . 2010,第002期

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5. AAV介导的HMGB1抗体基因转导及靶向LPS和HMGB1的抗体疗法治疗脓毒症小鼠的研究 [A] . 孙立柱 . 2021