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Process for production of virus DNA citoplasmico eucariotico modified virus DNA citoplasmico eucariotico modidicado, molecule of DNA.Set of components for direct molecular cloning of a genomaviral modified a virus DNA citoplasmico eucariotico, plasmideo and virus vaccine modified
Process for production of virus DNA citoplasmico eucariotico modified virus DNA citoplasmico eucariotico modidicado, molecule of DNA.Set of components for direct molecular cloning of a genomaviral modified a virus DNA citoplasmico eucariotico, plasmideo and virus vaccine modified
A method is disclosed for producing a modified eukaryotic cytoplasmic DNA virus by direct molecular cloning of a modified DNA molecule comprising a modified cytoplasmic DNA virus genome. The inventive method comprises the steps of (I) modifying under extracellular conditions a DNA molecule comprising a first cytoplasmic DNA virus genome to produce a modified DNA molecule comprising the modified cytoplasmic DNA virus genome; (II) introducing the modified DNA molecule into a first host cell which packages the modified DNA molecule into infectious virions; and (III) recovering from the host cell virions comprised of the modified viral genome. The host cell is infected with a helper virus which is expressed to package the modified viral genome into infectious virions. Examples of packaging a modified poxvirus genome by a helper poxvirus of the same or different genus are described. Also disclosed are novel poxvirus vectors for direct molecular cloning of open reading frames into a restriction enzyme cleavage site that is unique in the vector. In one model poxvirus vector, the open reading frame is transcribed by a promoter located in the vector DNA upstream of a multiple cloning site comprised of several unique cleavage sites.
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