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Methods to prepare highly Purified gamma globulin, free of ineffectiveness with hepatitis B virus
Methods to prepare highly Purified gamma globulin, free of ineffectiveness with hepatitis B virus
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机译:制备不含丙型肝炎病毒无效的高度纯化的丙种球蛋白的方法
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摘要
The present Invention relates to a method to eliminate the infectivity of hepatitis B with a Fluid Body that contains gamma glubilina,Characterized in that it comprises the steps of: a) providing the body fluid containing gamma globulin to Purify; (b) Implement the body fluid to a First and a Second columnAs in series, The First Column containing an effective amount of a DEAE - Sepharose Resin that is Rigid or DEAE bio gel (High Capacity)And the second column contains an effective amount of a Resin which consists of Rigid or DEAE - sephandez qae - sephandex; (c) to elute the body fluid of the first column with a Regulator SetOr at least at a pH of 7.0 and the Regulator approximately 0.If the Regulator 02 m phosphate Resin was selected for the second cvolumna is qae - sephandex, consisting of approximately 0.04 or Regulator Regulator m tris, 0.05 m Regulator about 0.035 m imidazole.Regulator of sodium phosphate (Na2HPO4 - Citric Acid), if the selected resin to the second DEAE sephandez or sephandez volumna of QAE,(d) monitor the effluent containing Proteins (f) Implement the collected effluent containing Proteins, the first column to the second.(g) to elute the Fluid of the second column, with one of the Regulators can be used in the first column, with the Regulator to a pH of at least 7.0 being the Regulators used in the first and second columns, respectively.The same or different and (H) to collect the effluent of the second column so that it is now eliminated any ineffectiveness with the hepatitis B virus.
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