METHOD OF CHROMOGENIC SUBSTRATE PREPARING FOR CHITINASE ACTIVITY DETERMINATION

摘要

Usage: Enzymology, microbiological industry as a substrate for determining chitinase activity in enzyme preparations and biological fluids. SUMMARY OF THE INVENTION: staining performed colloidal chitin or vinylsulfonic thiazine dye in a solution containing hydro- xylo-ions at a concentration of 0.1-0.25 M by incubating the reaction mixture at 100 & amp; deg; C for 10-30 min. When this dye at a concentration advantageously take 10-15 wt.%. 1 z.p.f BACKGROUND yl 4.