METHOD OF CHROMOGENIC SUBSTRATE PREPARING FOR CHITINASE ACTIVITY DETERMINATION

摘要

use: u044du043du0437u0438u043cu043eu043bu043eu0433u0438u00a0, u043cu0438u043au0440u043eu0431u0438u043eu043bu043eu0433u0438u0447u0435u0441u043au0430u00a0 industry as a substrate u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 u0445u0438u0442u0438u043du0430u0437u043du043eu0439 activity in enzyme preparations and u0431u0438u043eu043bu043eu0433u0438u0447u0435u0441 u043au0438u0445 environments.the essence of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0: u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 staining u043au043eu043bu043bu043eu0438u0434u043du043eu0433u043e u0445u0438u0442u0438u043du0430 u0442u0438u0430u0437u0438u043du043eu0432u044bu043c or u0432u0438u043du0438u043bu0441u0443u043bu044cu0444u043eu043du043eu0432u044bu043c dye in solution containing hydro u043au0441u0438u043b - ions in u043au043eu043du0446u0435u043d u0442u0440u0430u0446u0438u0438 0.1 to 0.25 m, by u0438u043du043au0443u0431u0438u0440u043eu0432u0430u043du0438u00a0 reaction mixture with 100 & deg; c for 10 to 30 minutes. the dye mainly take in a concentration of 10 - 15 mas.%. 1 u0437.u043f.u0444 - lu, 4).