1995, Oligonucleotides primers and oligonucleotides probes for the detection of pathogens of the buccal cavity.

摘要

Development of highly sensitive processes for the detection of anaerobic bacteria and microaerophilic pathogens of the buccal cavity from samples taken directly by the practitioner. Current research in odontology shows that anaerobic bacteria or gram-negative micro-aerophils play an important role in diseases of the buccal cavity, especially those affecting the paradentium. Research into these pathogens is important for the practitioner to make a precise diagnosis and adapt the therapeutic treatment. The invention disclosed herein consists of a process for taking and processing samples, amplifying the DNA contained in the latter by using an oligonucleotide primers for amplification of the DNA of the pathogens, followed by hybridization of the amplification product with an oligonucleotide probe specific to pathogens whose presence is to be detected.