Methods using novel chimeric transforming growth factor- 1/2

摘要

The present invention relates to the production of large quantities of a novel chimeric TGF-(beta), termed TGF-(beta)1/(beta)2, by eucaryotic host cells transfected with recombinant DNA vectors containing the TGF-(beta)1/(beta)2 precursor coding sequence controlled by expression regulatory elements. Simian TGF-(beta)1 cDNA (Sharples et al., 1987, DNA 6:239-244) was modified so that the nucleotides encoding amino acid residue numbers 9-13, 17, 19, 25 and 26 of the mature TGF-(beta)1 sequence were hanged to the nucleotides encoding the corresponding amino acids of the mature TGF-(beta)2 structure. Simian codon usage was maintained. The chimeric TGF-(beta)1/(beta)2 of the invention induces effects on the proliferation of vascular endothelial cells equivalent to those induced by TGF-(beta)1.