PREPARATION OF BACTERIUM FOR TRANFORMATION OF AGROBACTERIUM RHIZOGENES MAFF 03-01724 STRAIN

摘要

PURPOSE: To improve a method for preparing a transforming bacterium for introducing a foreign DNA into Agrobacterium rhizogenes MAFF 03-1724 strain with an improved efficiency. ;CONSTITUTION: In preparation of a bacterium required for transformation of domestic Agrobacterium rhizogenes MAFF 03-1724 strain by introducing a foreign DNA thereinto according to the electroporation method, this bacterium strain is cultured in a flask equipped with 2 to 10 buffles. When washing the cultured material with 10wt.% glycerol solution, the cultured material is carefully and repeatedly sucked and discharged using a 10ml Komagome type pipette 10 to 100 times so as to disperse lumped materials in the cultured material. Thereby, the objective transforming bacterium exhibiting a high efficiency of foreign-DNA introduction can be prepared.;COPYRIGHT: (C)1994,JPO&Japio