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ROSE PLANTS AND METHODS FOR THEIR PRODUCTION AND GENETIC TRANSFORMATION

机译:玫瑰植物及其生产和遗传转化方法

摘要

Somatic embryos and plantlets are produced from mature rose plant material by culturing in callus induction medium followed by exposure to regeneration medium. Rose plant cells are transformed by incubation with Agrobacterium cells carrying an exogenous DNA sequence. The callus cells may be obtained from various tissue sources, including stamen filaments, leaf explants, and the like, and whole rose plants may be regenerated from the transformed callus cells. The exogenous DNA will be stably incorporated into the chromosomes of the regenerated rose plant which will be able to express gene(s) encoded by the DNA sequence.
机译:通过在愈伤组织诱导培养基中培养,然后暴露于再生培养基,从成熟的玫瑰植物材料产生体细胞胚和小植株。通过与带有外源DNA序列的农杆菌细胞一起孵育来转化玫瑰植物细胞。愈伤组织细胞可获自各种组织来源,包括雄蕊丝,叶外植体等,并且可从转化的愈伤组织细胞再生完整的月季植物。外源DNA将被稳定地整合到再生玫瑰植物的染色体中,该染色体将能够表达由DNA序列编码的基因。

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