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METHOD OF DEOXYRIBONUCLEIC ACID HYBRIDIZATION
METHOD OF DEOXYRIBONUCLEIC ACID HYBRIDIZATION
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机译:脱氧核糖核酸加氢的方法
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摘要
The invention relates to the field of molecular biology for research and diagnostic purposes can be utilized. The purpose of the invention - method To achieve simplification purpose are microporous nylon membrane with a pore size of 02 microns production plant Himfil p / k Hin Kaluri to which is applied the DNA to be analyzed in a solution containing 0-0.1 M NaCI or NH / Ac membranes were irradiated with ultraviolet light at a dose of 0.6 - 0.8 kJ / m2 at a wavelength of 260 nm, and incubated with probe DNA in a solution containing 0,5% SDS.
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机译:本发明涉及用于研究和诊断目的的分子生物学领域。本发明的目的-方法为了实现简化目的,是孔径为02微米的微孔尼龙膜生产工厂Himfil p / k Hin Kaluri,在含有0-0.1M NaCl或NH 3的溶液中向其施加待分析的DNA。以0.6-0.8kJ / m 2的紫外线在260nm的波长下对Ac / Ac膜进行照射,并在含有0.5%SDS的溶液中与探针DNA一起孵育。
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