Method for Increasing the Hybridization Efficiency of DNA (Deoxyribonucleic Acid) and RNA (Ribobonucleic Acid) Probes

摘要

A proprietary system has been developed which increases the hybridization efficiency of radiometric probes by two orders of magnitude. The system was applied to commercially available systems for the non-radiometric detection of DNA sequences, based on the incorporation and detection of biotinylated nucleotides. Under the appropriate hybridization conditions, detection of 5 picograms of viral DNA, equivalent to 100,000 viral particles, was consistently obtained with a 16-hour hybridization reaction; 25 picograms or 500,000 viral particles could be detected in one working day by shortening the hybridization to 4 hours. These results represent a significant increase in the sensitivity of non-radiometric DNA detection systems, and increase the feasibility of using DNA-DNA hybridization reactions for clinical diagnosis of disease organisms or genetic abnormalities.