This invention is in the field of glucose isomerization enzymes. More specifically, the invention is directed to a novel xylose isomerase, a process for the preparation of this enzyme, the use of this enzyme in glucose isomerization processes, and glucose isomerization processes. PPThe enzyme is preferrably derived from Thermotoga maritima or Thermotoga neapolitana. The enzyme has a temperature optimum above 90. degree. C., pH optimum in the range of from 6 to 7 and a residual activity at 90° C. of more than 40% after 30 minutes and/or residual activity at 98° C. of more than 20% after 30 minutes. The enzyme can also be in immobilized form.
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