Antibodies specific for either ras photo-oncogene encoded P21 proteins or ras oncogene encoded P21 proteins but not for both and method of producing same

摘要

Experiments designed to define the differences between the pas p21 oncogene DNA isolated from human bladder cancer cells and its corresponding proto-oncogene are described herein. By a series of in vitro recombinations, the difference was initially isolated to a 350 kb segment of DNA; sequencing defined the difference as a change in the Gly. sup.12 codon causing the p21 protein of the oncogene to contain valine at a location where the p21 protein of the proto-oncogene contained glycine. Assays for detecting carcinogenesis based on such differences are also described. In one type of assay, a restriction enzyme specific for either the altered or non-altered DNA segment of the genes are employed to detect carcinogenesis. In another type of assay, serological reagents, such as antibodies specific for either p21 protein expressed from the proto-oncogene or p21 expressed from the oncogene, or a common site therein, are described.