UNITARY TESTING TOOL FOR SCREENING GRAM- NEGATIVE-BACTERIUM URINE DISEASE
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机译:筛查革兰氏阴性菌尿病的通用测试工具
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摘要
PURPOSE: To make rapid and convenient group screening possible by containing a test composition, composed of lysate, bivalent positive ion synthetic substrate, a buffer component and a lysate stabilizing component, in a carrier matrix for preparation. CONSTITUTION: Lysate is obtained in a frozen dry form, and calcium positive ion is desirable as bivalent positive ion required for the activation of lysate cascade. Luminous synthetic substrate is desirable as synthetic substrate, and endotoxin LAL testing fluorescent substrate with an elimination radical is used. High-quality filter paper is desirable as a carrier matrix. A reagent solution is impregnated into the filter paper, dried, cut and stuck to a support body member. A testing instrument using this member does not respond to free endotoxin but shows sensitivity to 105 Gram-negative bacteria per 1ml of urine. For preparation, a buffer component of pH7.5-8.5 such as trisaminomethane is contained, and it is further necessary to add a stabilizing reagent for proenzyme in the lysate, for instance, a polymer or the like charged to neutral.
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