A method for purifying two isozymes of Candida rugosa lipase.

摘要

PROCEDURE FOR THE CLEANSING OF TWO ISOENZYMES Candida rugosa lipase. A method for purifying THESE TWO LIPASES EXTRACELLULAR lipase COMMERCIAL GROSS rugosa. The method comprising a single step CROMATOGRAFIA Hydrophobic IN agarose matrix. VERY DIFFERENT ELUTION CONDITIONS OF PERMIT FULL TWO SEPARATION AND PURIFICATION OF ACTIVITY Lipase enzymes (lipases AYB) PRESENT IN THE COMMERCIAL starting extract. IS USED THEREFOR BUFFER CONCENTRATE pH 6 to 8, with which elute from the column much of the contaminants present in the extract; Then with the same buffer solution more dilute CRAWLING THE LIPASE B. THEREAFTER WITH DI O POLIALCOHOL dissolved in buffer elutes THE DILUTED lipase, whose solution by concentration by ultrafiltration and gel permeation chromatography MOLECULAR dextran, REMOVED THE ALCOHOL. APPLIED industrially enzyme purification.