皱褶假丝酵母Candida rugosa产脂蛋白酯酶的发酵培养基优化

摘要

Candida rugosa producing Iipoprotein lipase was screened, and the optimization of fermentation conditions was studied. The optimal medium compositions were as follows: olive oil 0.6%, proteose-peptone 0.5%, yeast extract 0.3%, malt extract 0.3%, Tween-80 0.1%, K2HPO4 0.05%, Na2HPO4 0.1%, MgSO4·7H2O 0.1%. Under the optimum cultivation conditions, the enzyme activity reached 121.65U/L%将筛选得到的产脂蛋白酯酶的皱褶假丝酵母进行了底物诱导和发酵培养基的优化,确定了其最适培养基配方:橄榄油0.6％,蛋白胨0.5％,酵母膏0.3％,麦芽浸膏0.3％,Tween-80 0.1％,K2HPO4 0.05％,Na2HPO4 0.1％,MgSO4·7H2O 0.1％.在最适培养基中培养,脂蛋白酯酶的酶活力可达121.65U/L,比未优化前提高了80倍.