Method for the immuno-assay of antithrombin iii activated by a glycosaminoglycan, corresponding monoclonal antibodies and method for producing them

摘要

PCT No. PCT/FR95/00456 Sec. 371 Date Oct. 11, 1996 Sec. 102(e) Date Oct. 11, 1996 PCT Filed Apr. 10, 1995 PCT Pub. No. WO95/28644 PCT Pub. Date Oct. 26, 1995A method that assays activated antithrombin III (AT III) is useful for monitoring the effect in vivo of an agent that activates AT III. It involves the steps of contacting AT III with a solution sample containing the agent, wherein the agent is a sulfated glycosaminoglycan or a sulfated oligosaccharide, followed by contacting the sample with i) a first monoclonal antibody that preferentially binds AT III activated by the agent, as opposed to binding native AT III, and ii) a second monoclonal antibody that preferentially binds denatured AT III as opposed to either AT III activated by the agent or native AT III.