METHOD FOR THE IMMUNO-ASSAY OF ANTITHROMBIN III ACTIVATED BY A GLYCOSAMINOGLYCAN, CORRESPONDING MONOCLONAL ANTIBODIES AND METHOD FOR PRODUCING THEM

摘要

The invention relates to a method for determining the antithrombin III (AT III) activated by a sulfated glycosaminoglycan or a sulfated oligosaccharide whose in vivo effect on AT III is similar to that of heparin, by an immuno-assay of the sandwich type in solid phase, characterized in that the method implements: a monoclonal antibody which recognizes, in solution, AT III less efficiently than AT III activated by a sulfated glycosaminoglycan or one of the sulfated oligosaccharides, having an activity on AT III similar to that of heparin; and a monoclonal antibody which substantially does not recognize native AT III in solution and AT III activated by one of said sulfated glycosaminoglycans or said oligosaccharides in solution, but which recognizes denaturated AT III.