Improved production method of culture medium for pneumococcus and composition of polysaccharide of pneumococcus

摘要

The present invention relates to a medium containing a casein hydrolyzate optimized for the cultivation of pneumococcus, and an improved method for producing a pneumococcal polysaccharide vaccine by culturing pneumococci under aeration using the medium. The culture medium according to the present invention is prepared by adding 0.5 to 1.5 w / v% of casein hydrolyzate, 0.1 to 1.0 w / v% of tryptone, 0.1 to 1.5 w / v% of sodium chloride, 0.1 to 0.5 w / v% of yeast extract, (K₂HPO₄), 0.2 to 2.0 w / v% of a carbon source, and optionally 1 to 10 mM of a metal ion source and 0.1 to 1000 ppm of an amino acid.;Using the culture medium according to the present invention, the antigenic specificity is not changed at all under aerobic condition of pneumococcus, and the desired antigenic capsular polysaccharide can be produced at a high yield.