Recombinant DNA, expression vector and plasmid, production method thereof and inducible and repressible foreign gene expression method

摘要

A recombinant DNA contains the consensus sequence …IMAGE… and a promoter which has the DNA sequence GGN10GC and is obtainable by identifying a DNA sequence containing the consensus sequence from a gene bank of a microorganism which contains a gene which is repressible by O2 and inducible under anaerobic conditions by formate according to known methods, isolating it and combining it with a suitable promoter. This recombinant DNA is suitable for the inducible and repressible expression of a heterogeneous gene by effecting induction under anaerobic conditions by means of formate and repression by means of O2. …IMAGE…