HCV ANTI-SENSE RNA, MANIFESTATION VECTOR CONTAINING THE SAME, AND THERAPEUTIC AGENT FOR HCV-INVOLVED DISEASE CONTAINING THE RNA OR MANIFESTATION VECTOR

摘要

PURPOSE: To obtain the subject new RNA having anti-sense activity of hepatitis C virus (HCV) genome to 5'-nontranslational region, inhibiting in vivo HCV proliferation through suppressing the manifestation of HCV's structural protein, thus useful as e.g. a therapeutic agent for HCV-involved diseases. ;CONSTITUTION: This new HCV anti-sense RNA has a nucleotide sequence of formula I, II, III, IV, V or VI, having anti-sense activity of HCV genome to a fractional sequence of 5'-non-translational region and inhibiting in vivo proliferation of HCV through suppressing the manifestation of HCV's structural gene, thus being useful as a therapeutic agent for HCV-involved diseases such as hepatitis, hepatocirrhosis and hepatoma. This anti-sense RNA is obtained by transfer reaction, using a RNA polymerase, of a template DNA, the aimed amplified nucleotide sequence produced by polymerase chain reaction using a set of primers suitable for obtaining the aimed nucleotide sequence complementary to the fractional sequence of HCV's 5'-non-translational region.;COPYRIGHT: (C)1995,JPO