RECOMBINANT VECTORS DERIVED FROM ADENOVIRUS FOR USE IN GENE THERAPY

摘要

The invention provides novel vectors derived from adenovirus which are specifically suitable for use in methods of gene therapy. The vectors have a deletion compared to wild-type adenovirus in that the E1 region is not present in a functional manner. Additionally the vectors are characterized in that they do contain a part of the E3 region of adenovirus which is biologically functional. This results in vectors which do normally not lead to expression of adenoviral proteins, but which in the cases where such proteins do become expressed will repress the host's immunological response to said proteins. Thereby host cells infected (provided) with the vector will live longer. Therefore, the product introduced into said cells providing the therapy will be produced in larger amounts, or at least for a prolonged period. Preferably the vector comprises genetic information for antisense therapy, or for genes which will combat tumors, such as genes encoding cytokines, preferably IL-1, or so called suicide genes, such as Herpes Simplex Virus thymidine kinase. Methods for producing said vectors and methods of gene therapy or uses therein are also disclosed.