Lipase immobilized on a chitosan carrier

摘要

An enzyme immobilizing carrier is produced by dissolving low- molecular weight chitosan in an aqueous acid solution and dropping the solution into a basic solution to produce regenerated porous chitosan in particles, reacting the regenerated porous chitosan in particles with the glycidyl ether of an aliphatic polyalcohol, and reacting further the resulting chitosan with the acid halide or acid anhydride of a higher fatty acid in a polar organic solvent. An enzyme and a polyfunctional cross-linking agent are reacted with the carrier to covalently immobilize the enzyme on the carrier. In a preferred embodiment, the enzyme is lipase and the carrier is produced by introducing the glycidyl ether of an aliphatic polyalcohol at 0.01 to 0.4 mole to 1 mole of the pyranose ring residue of the chitosan and by introducing a higher fatty acid having a total carbon number of 6 to 20 at 0.05 to 1 mole to 1 mole of the pyranose ring residue of the chitosan.